Characterization of mouse lymphohematopoietic stem cells lacking spleen colony-forming activity

Characterization of mouse lymphohematopoietic stem cells lacking spleen colony-forming activity.

The classical definition of lymphohematopoietic stem cells (LHSC), the most primitive progenitors of all blood cells, requires that they have the capacity for self-renewal and for the long-term production of all blood cell lineages. However, other characteristics of LHSC have been debated. Our previous data suggested that mouse LHSC are very slowly proliferating cells that generate delayed mult...

The effect of corticotropin on mouse spleen colony-forming cells.

Several investigators have reported a retechnique. There was a marked decrease duction in the number of colony-forming in spleen CPU in these mice when cornunitsjCFU) in the sple.ns of mice exposed pared with control animals, but marrow to hypoxia and have postulated that this is CPU were not reduced. These tesults mdis.condary to a direct toxic effect of hypoxcafe that part of the effect of hy...

Fractionation of mouse bone marrow by adherence separates primitive hematopoietic stem cells from in vitro colony-forming cells and spleen colony-forming cells.

Fractionation of mouse bone marrow by adherence to tissue culture plastic was used to characterize the adhesive properties of hematopoietic stem (HS) cells capable of long-term reconstitution. The adherent fraction that represents approximately 13% of the total marrow population was virtually devoid of in vitro colony-forming cells and spleen colony-forming cells but did contain approximately 3...

Separation of erythropoietin-sensitive cells from hemopoietic spleen colony-forming stem cells of mouse fetal liver by unit gravity sedimentation.

A Stochastic Model of Stem Cell Proliferation, Based on the Growth of Spleen Colony-forming Cells.

Normal mammalian hemopoietic tissue produces a continuous supply of differentiated blood cells whose functions are essential for life. These functional cells have limited life spans and are incapable of self-renewal. For example, erythrocytes lack nuclei and thus, though admirably adapted to their function of gas transport, are incapable of mitosis. Similarly, lack of proliferative capacity is ...